منابع مشابه
Quantitative Fluorescence Microscopy
Printed in the Netherlands " You have walked to the edge of the great mosaic of human knowledge. Up until now, you have been living in a world full of ideas and concepts that other people have set out for you. Now it is your turn. You get to design a piece of the mosaic and glue it down. It just has to fit with what else is there. And if you do a good job shaping your tile, it will be easier fo...
متن کاملQuantitative fluorescence microscopy: from art to science.
A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present i...
متن کاملAccuracy and precision in quantitative fluorescence microscopy
The light microscope has long been used to document the localization of fluorescent molecules in cell biology research. With advances in digital cameras and the discovery and development of genetically encoded fluorophores, there has been a huge increase in the use of fluorescence microscopy to quantify spatial and temporal measurements of fluorescent molecules in biological specimens. Whether ...
متن کاملAutomated quantitative live cell fluorescence microscopy.
Advances in microscopy automation and image analysis have given biologists the tools to attempt large scale systems-level experiments on biological systems using microscope image readout. Fluorescence microscopy has become a standard tool for assaying gene function in RNAi knockdown screens and protein localization studies in eukaryotic systems. Similar high throughput studies can be attempted ...
متن کاملQuantitative colocalization analysis of confocal fluorescence microscopy images.
Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of images with colocalization based on the calculation of a number of specialized coefficients. First, images of double-stained sections are subjected to background correction. Then, various coefficients are calculated. Meanings of the coefficients and a guide to in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of The Royal Society Interface
سال: 2008
ISSN: 1742-5689,1742-5662
DOI: 10.1098/rsif.2008.0500.focus